akt  (Cell Signaling Technology Inc)

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: PC capsules promoted diabetes skin wound healing via activating PI3K/AKT signaling pathway: (A) top 20 KEGG pathway (signal transduction) analysis of differential genes between control group and PC capsule treatment group; (B) gene set enrichment analysis of regulated gene pathways with KEGG and Genomes databases; (C–E) IHC results showing increase in expression of p-PI3K in PC capsule group. Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: #p < 0.05 (vs control group); ∗p < 0.05, ∗∗p < 0.01 (vs PC group).

Article Snippet: After blocking (5% skim milk/bovine serum albumin, 1.5 h), the membranes were incubated overnight at 4 °C with the primary antibodies against p-AKT (#4060, CST, USA; 1:1000), p-PI3K (#AF3241, Affinity, China; 1:1000), total AKT (#2920, CST, USA; 1:1000), and PI3K (#4249, CST, USA; 1:1000).

Techniques: Capsules, Transduction, Control, Expressing, Standard Deviation

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: PC capsule–mediated cytoprotection via promoting activation of PI3K/AKT signaling pathway: expression of p-PI3K, PI3K, p-AKT, and AKT in HUVECs treated with PC capsules for 24 h and presence/absence of H 2 O 2 for 6 h (A); and analysis of optical density values of p-PI3K/PI3K (B) and p-AKT/AKT (C); expression of p-PI3K, PI3K, p-AKT, and AKT in NIH/3T3 cells treated with PC capsules for 24 h and presence/absence of H 2 O 2 for 6 h (D); and analysis of optical density values of p-PI3K/PI3K (E) and p-AKT/AKT (F). Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: #p < 0.05, ##p < 0.01, ###p < 0.001 (vs control group); ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (vs PC group or vs H 2 O 2 group).

Article Snippet: After blocking (5% skim milk/bovine serum albumin, 1.5 h), the membranes were incubated overnight at 4 °C with the primary antibodies against p-AKT (#4060, CST, USA; 1:1000), p-PI3K (#AF3241, Affinity, China; 1:1000), total AKT (#2920, CST, USA; 1:1000), and PI3K (#4249, CST, USA; 1:1000).

Techniques: Activation Assay, Expressing, Capsules, Standard Deviation, Control

Journal: Materials Today Bio

Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice

doi: 10.1016/j.mtbio.2026.103029

Figure Lengend Snippet: PC capsules improve HUVEC and NIH/3T3 cell dysfunction by regulating PI3K/AKT signaling. HUVECs and NIH/3T3 cells were treated with PC capsules and LY294002 for 24 h and H 2 O 2 for 6 h. (A) Images of HUVEC migration exposure to H 2 O 2 , LY294002, PC capsules after 24 h; (B) quantification of percentage wound area remaining for HUVECs; (C) images of NIH/3T3 cell migration exposure to H 2 O 2 , LY294002, PC capsules after 24 h; (D) quantification of percentage wound area remaining for NIH/3T3 cells; (E–F) digital images of endothelial cell microtubule formation after treatment with H 2 O 2 , LY294002, and PC capsules. Quantification of (G) number of branch sites, (H) total lengths and (I) numbers of tube nodes (J); and analysis of optical density values of p-PI3K/PI3K (K) and p-AKT/AKT (L); expression of p-PI3K, PI3K, p-AKT, and AKT in NIH/3T3 cells treated with PC capsules, and LY294002 for 24 h and presence/absence of H 2 O 2 for 6 h (M); and analysis of optical density values of p-PI3K/PI3K (N) and p-AKT/AKT (O). Data are presented as mean ± standard deviation (SD) with n = 3 independent biological replicates per group. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Significance markers are defined as: ##p < 0.01, ###p < 0.001 (vs. control group); ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (vs PC group or vs LY294002 group).

Article Snippet: After blocking (5% skim milk/bovine serum albumin, 1.5 h), the membranes were incubated overnight at 4 °C with the primary antibodies against p-AKT (#4060, CST, USA; 1:1000), p-PI3K (#AF3241, Affinity, China; 1:1000), total AKT (#2920, CST, USA; 1:1000), and PI3K (#4249, CST, USA; 1:1000).

Techniques: Capsules, Migration, Expressing, Standard Deviation, Control